Certificate of HPLC Practice

  • This course is designed for beginners and intermediate-level users in HPLC who want practical laboratory experience in the PSCC. The lectures, supplemented by practice, problems sets, slides, and video tapes, provide the fundamentals needed to understand the techniques and instrumentation involved in this powerful analytical tool. No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course. Some experience in HPLC or introductory course work in organic chemistry is desirable but not necessary.
    • Basic instrumentation for HPLC
        Detectors: UV/VIS, photo diode array, mass spectrometer, fluorescence
        Column selection and optimization
        Qualitative and quantitative analysis
        Troubleshooting HPLC systems
  •   Learn about basic instrumentation for HPLC
      Learn about detectors: UV/VIS and PDA
      Become familiar with column selection and optimization
      Perform qualitative and quantitative analysis
      Understand how to troubleshoot HPLC systems
  •  HPLC Overview
        Introduction to HPLC, instrumentation overview, detector details and laboratory:
        basic HPLC hardware

      Separation Fundamentals and Mobile Phase
        Practical HPLC theory, important HPLC parameters, the mobile phase and laboratory:
        mobile phase strength flow rate and temperature

     Separation Modes and Columns
       Reversed phase, normal phase, ion exchange, size exchange and laboratories:
       pH effects in reversed phase and column dimensions.

     Quantitative Analysis and Troubleshooting
       Qualitative and quantitative analysis, hardware troubleshooting, separation
       troubleshooting and laboratory: internal vs. external standard; hardware troubleshooting

     Gradients and Method Development
       Gradients; fundamentals of method development; laboratory:
       develop a method from a completely unknown mixture