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HPLC Practice

This course is designed for beginners and intermediate-level users in HPLC who want practical laboratory experience in the PSCC.






Financial aid may be available to those who qualify


• Markham
• North York

Program Description

This course is designed for beginners and intermediate-level users in HPLC who want practical laboratory experience in the PSCC. The lectures, supplemented by practice, problems sets, slides, and video tapes, provide the fundamentals needed to understand the techniques and instrumentation involved in this powerful analytical tool. No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course. Some experience in HPLC or introductory course work in organic chemistry is desirable but not necessary.
Key Topics of This Dynamic Course

Basic instrumentation for HPLC
Detectors: UV/VIS, photo diode array, mass spectrometer, fluorescence
Column selection and optimization
Qualitative and quantitative analysis
Troubleshooting HPLC systems

How You'll Benefit from This Course

Learn about basic instrumentation for HPLC
Learn about detectors: UV/VIS and PDA
Become familiar with column selection and optimization
Perform qualitative and quantitative analysis
Understand how to troubleshoot HPLC systems

Course Topics

HPLC Overview
Introduction to HPLC, instrumentation overview, detector details and laboratory:
basic HPLC hardware

Separation Fundamentals and Mobile Phase
Practical HPLC theory, important HPLC parameters, the mobile phase and laboratory:
mobile phase strength flow rate and temperature

Separation Modes and Columns
Reversed phase, normal phase, ion exchange, size exchange and laboratories:
pH effects in reversed phase and column dimensions.

Quantitative Analysis and Troubleshooting
Qualitative and quantitative analysis, hardware troubleshooting, separation
troubleshooting and laboratory: internal vs. external standard; hardware troubleshooting

Gradients and Method Development
Gradients; fundamentals of method development; laboratory:
develop a method from a completely unknown mixture


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